分阶洗脱
Through 2-Stepwise elution chromatography , tea polyphenols ( Tp ) and caffeine were separated from tea with macroporous resin.
茶叶提取液经大孔吸附树脂二级阶段洗脱 层析分离 茶多酚咖啡碱.
SISE p1 was further fractionated by DEAE cellulose ion exchange chromatography and four fractions were obtained by stepwise elution. The fraction eluted with Tris HCl buffer containing 0.2 mol of sodium chloride per liter had the highest activity.
用DEAE-纤维素离子交换层析对SISE-p1进行分离,生物活性研究表明用含0.2mol/L氯化钠的Tris-HCl缓冲液(pH7.0)洗脱的组分具有较强的生物活性。
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